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bacteria test instructions 1. Take out the Bacteria Test vial and set upright on a flat surface. 2. Collect water sample or turn on tap to a very slow stream. 3. Carefully twist off cap and fill vial to 1/2 inch below the top (to 5 ml line). DO NOT OVERFILL and DO NOT SPILL the bacterial growth powder in the vial. 4. Replace the cap and twist on tightly. Shake the vial vigorously for 20 seconds. 5. Place the capped vial upright in a warm area (70-90°F) where it cannot be disturbed for 48 hrs. 6.