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manual abstract
lid is fully seated). 2 Empty filtrate container. 2 Insert assembled concentrator into centrifuge (when fixed 3 Refill concentrator with an appropriate buffer. angle rotors are used, angle the concentrators so that the 4 Concentrate the sample again and repeat the printed windows face upwards/outwards). process until the concentration of the 3 Centrifuge at recommended speeds, taking care not to exceed contaminating species is sufficiently reduced. the maximum g force indicated in the table above (20 minutes Typically three wash cycles will remove 99% of at 7,500 g is recommended for 30 concentration of 4 mL at initial salt content. 10C). 4 Once the desired concentration is achieved, remove assembly See Notes on next page. and recover sample from the bottom of the concentrate pocket with a pipette. Equipment required 4 mL Spin Concentrators, pack of 25 Centrifuge Rotor type Minimum rotor angle Rotor cavity Minimum effective speed Recommended speed Maximum speed Concentrate recovery Pipette type Recommended tip Swing bucket rotor Fixed angle rotor Total Length Width Active Membrane Area Hold-up volume of membrane Dead stop volume pH range Materials of construction Body Filtrate vessel Concentrator cap Membrane Instructions – 15 mL Concentrators 30,000 Molecular Weight Cut-Off (MWCO) 15 mL Spin Concentrator, Part No. 5188-2799 Equipment required 15 mL Spin Concentrators, pack of 10 Centrifuge Rotor type Swing bucket or Fixed angle Minimum rotor angle 25° Rotor cavity To fit 30 x 114 mm conical bottom tubes or 29 x 104 mm round bottom tubes (50mL) Minimum effective speed 1,000 g Recommended speed 2,000 g Maximum speed 3,000 g Concentrate recovery Pipette type Fixed or variable volume Recommended tip Thin gel loader type Technical specifications Concentrator sample volume capacity Swing bucket rotor 2-15 mL Fixed angle rotor 2-8 mL Dimensions Total Length 76 mm Width 25.5 mm Active Membrane Area 4 cm2 Hold-up volume of membrane <20 .L Dead stop volume 50 .L pH range pH 1 – 9 Materials of construction Body Polycarbonate Filtrate vessel Polypropylene Concentrator cap Polycarbonate Membrane Polyethersulfone Concentrating Proteins with 15 mL Concentrators: 1 Fill concentrator with sample within the working volume capacity range noted in the table above, taking care not to touch the membrane surface, and then close the hinged lid (ensure that the lid is fully seated). 2 Place concentrator into 50 mL centrifuge tube (filtrate collection vessel – not supplied, user must provide). 3 Insert assembled concentrator into centrifuge (when fixed angle rotors are used, angle the concentrators so that the printed windows face upwards/outwards). Spin at recommended speeds, taking care not to exceed the maximum g force indicated in the table above (20-40 minutes is common). 4 Once the desired concentration is achieved, remove assembly and recover sample from the bottom of the concentrate pocket with a pipette. Desalting/Buffer Exchange See 4 mL Spin Concentrator instr...
Other models in this manual:Network Equipment - 5185-5991 (262.21 kb)